Influência da ativação ultrassônica do ácido glicólico na penetração do irrigante nos túbulos dentinários através da microscopia confocal a laser - estudo in vitro

Abstract

This study aims to evaluate, in vitro, the influence of the ultrasonic activation of glycolic acid on the penetration of the irrigant into the dentinal tubules through confocal laser microscopy. Thirty mandibular premolars were obtained and the coronal portion was sectioned so that the remaining root was 15 mm. They were instrumented with Reciproc R25 (VDW), the final wash was performed with 5ml of saline solution and dried with absorbent paper (Dentsply Maillefer), and every 5ml of final irrigant was marked with rhodamine B fluorescent isothiocyanate at 0 .1% by weight (Sigma-Aldrich, St Louis, MO). The samples were divided into 3 groups, following the final irrigation: distilled water, 17% glycolic acid and 17% glycolic acid + US, for a period of 1 minute each protocol. After completing the protocols, all canals were aspirated and dried with absorbent paper cones (Dentsply Maillefer) and sectioned with 500 μm thickness. Samples were examined by a confocal laser microscope (Zeiss LSM-Pascal; Carl Zeiss, Goëttingen, Germany). Digital images were imported into the ImageJ program (ImageJ software, NIH) for analysis of penetration into the dentinal tubule. Statistical analysis was performed using ANOVA and Tukey's Post–Hoc tests (α= 0.05). The results showed a statistically significant difference between the ultrasound group and the other groups (p<0.05). It is concluded that the use of ultrasonic activation on glycolic acid increases the ability of the irrigant to penetrate tubular dentin.