Avaliação da efetividade antimicrobiana de diferentes agentes químicos na eliminação de Enterococcus faecalis e Candida albicans

Abstract

The objective of the present study was to evaluate the antimicrobial effectiveness of sodium hypochlorite and calcium hypochlorite in different concentrations (1%, 2.5%, 5.25%) comparing them to chlorhexidine 2%, in the elimination of Enterococcus faecalis and Candida albicans. Pure cultures of E. faecalis and C. albicans were obtained from the Microbiology Laboratory of the Institute of Biological Sciences at UPF. Microbial suspensions of the two cultures were prepared in a laminar flow hood and standardized to tube number 10 on the MacFarland MacFarland scale (concentration of 3,106 cells/mL). After 0.5 mL of each suspension were poured into Petri dishes with Mueller-Huinton culture medium (for E. faecalis) and Saboraud agar (for C. albicans). Sterile paper discs were placed on the cultures, where 20 uL of the test substances (sodium hypochlorite and calcium hypochlorite at concentrations of 1%, 2.5% and 5.25% and 2% chlorhexidine) were applied separately. , according to concentration and in duplicates. The cultures were incubated for 48 hours at 37C in a bacteriological oven. E. faecalis was more resistant to all tested products when compared to C. albicans. At concentrations of 1% and 2.5%, calcium hypochlorite showed greater antimicrobial activity than sodium hypochlorite, but a lower capacity to eliminate this microorganism compared to 2% chlorhexidine. At a concentration of 5.25%, sodium hypochlorite was more effective against the microorganisms tested. It concluded that 2% chlorhexidine was the most effective product against E. faecalis. Against C. albicans, 5.25% sodium hypochlorite was more effective, followed by 2% chlorhexidine and 2.5% calcium hypochlorite. With the exception of calcium hypochlorite at a concentration of 5.25%, this product showed superior antimicrobial action on the microorganisms tested compared to sodium hypochlorite.